Molecular Characterization of Aquaporins Genes from the Razor Clam Sinonovacula constricta and Their Potential Role in Salinity Tolerance

Aquaporins (AQPs) play crucial roles in osmoregulation, but the knowledge about functions of AQPs Sinonovacula constricta is unclear. In this study, Sc-AQP1, Sc-AQP8, and Sc-AQP11 were identified from S. constricta, three Sc-AQPs are highly conserved compared to known AQPs. The qRT-PCR analysis revealed that highest mRNA expressions detected gill, digestive gland, adductor muscle, respectively. addition, expression Sc-AQP1 was D-shaped larvae stage, whereas SC-AQP8 observed umbo stage. significantly increased 12.45-, 12.36-, 27.44-folds post-exposure low salinity (3.5 psu), while only high (35 psu) (p < 0.01). fluorescence situ hybridization also showed shift led boost gill filament, Knockdown Sc-AQP8 decreased osmotic pressure hemolymph. Overall, these findings would contribute comprehension osmoregulation pattern constricta.